Title : Analytical cation-exchange chromatography to assess the identity, purity, and N-terminal integrity of human lactoferrin.

Pub. Date : 2002 Oct 1

PMID : 12381362






3 Functional Relationships(s)
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1 The elution profile of hLF on Mono S unequivocally differed from lactoferrins from other species as well as homologous and other whey proteins. Mono-S HLF transcription factor, PAR bZIP family member Homo sapiens
2 Mono S chromatography of 2 out of 26 individual human whey samples showed a rare polymorphic hLF variant with three N-terminal arginines (Gly(1)-Arg(2)-Arg(3)-Arg(4)-Ser(5)-) instead of the usual variant with four N-terminal arginines (Gly(1)-Arg(2)-Arg(3)-Arg(4)-Arg(5)-Ser(6)-). Mono-S HLF transcription factor, PAR bZIP family member Homo sapiens
3 In conclusion, Mono S cation-exchange chromatography appeared a robust method to assess the identity, purity, N-terminal integrity, and the presence of polymorphic and intact hLF variants. Mono-S HLF transcription factor, PAR bZIP family member Homo sapiens