Pub. Date : 2002 Sep
PMID : 12365719
7 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Prednisolone induces interleukin-18 expression in mononuclear blood and myeloid progenitor cells. | Prednisolone | interleukin 18 | Homo sapiens |
| 2 | METHODS: IL-18 transcript and protein levels in PBMC from untreated and prednisolone treated RA patients, and from healthy donors were assessed by semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR) and immunoblotting. | Prednisolone | interleukin 18 | Homo sapiens |
| 3 | IL-18 regulation was determined in PBMC and U937 cells upon exposure to prednisolone in vitro by RT-PCR and Northern Blot analysis, by ELISA in cell culture supernatants, and in transiently transfected THP-1 cells by IL-18 promoter activity luciferase assays. | Prednisolone | interleukin 18 | Homo sapiens |
| 4 | RESULTS: In RA PBMC, IL-18 transcript levels were dose dependently restored, in parallel with administered prednisolone treatment, to subnormal levels. | Prednisolone | interleukin 18 | Homo sapiens |
| 5 | In cultured PBMC and promonocytic cell lines, prednisolone up-regulated IL-18 transcription in parallel with increasing the IL- 18 protein release into cell culture supernatants. | Prednisolone | interleukin 18 | Homo sapiens |
| 6 | In cultured PBMC and promonocytic cell lines, prednisolone up-regulated IL-18 transcription in parallel with increasing the IL- 18 protein release into cell culture supernatants. | Prednisolone | interleukin 18 | Homo sapiens |
| 7 | CONCLUSION: Prednisolone increases IL-18 expression and release in PBMC and monocytic cell lines. | Prednisolone | interleukin 18 | Homo sapiens |