Pub. Date : 2002 Nov 1
PMID : 12193607
5 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Photoaffinity labeling with a specific probe, [(32)P]nicotinamide 2-azidoadenosine dinucleotide, was used to identify the NAD(+) binding site within human GDH encoded by the synthetic human GDH gene and expressed in Escherichia coli as a soluble protein. | NAD | glutamate dehydrogenase 1 | Homo sapiens |
| 2 | Photoaffinity labeling with a specific probe, [(32)P]nicotinamide 2-azidoadenosine dinucleotide, was used to identify the NAD(+) binding site within human GDH encoded by the synthetic human GDH gene and expressed in Escherichia coli as a soluble protein. | NAD | glutamate dehydrogenase 1 | Homo sapiens |
| 3 | Amino acid sequencing and compositional analysis identified Glu(279) as the site of photoinsertion into human GDH, suggesting that Glu(279) is located at or near the NAD(+) binding site. | NAD | glutamate dehydrogenase 1 | Homo sapiens |
| 4 | The K(m) values for NAD(+) were 10-14-fold greater for the mutant GDHs than for wild-type GDH, whereas the V(max) values were similar for wild-type and mutant GDHs. | NAD | glutamate dehydrogenase 1 | Homo sapiens |
| 5 | The results with photoaffinity labeling and cassette mutagenesis studies suggest that Glu(279) plays an important role for efficient binding of NAD(+) to human GDH. | NAD | glutamate dehydrogenase 1 | Homo sapiens |