Pub. Date : 2002 Apr 26
PMID : 12054585
9 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | In isolated pancreatic islets, alloxan almost completely blocked both glucosamine-induced and STZ-induced protein O-GlcNAcylation, suggesting that alloxan indeed was inhibiting (OGT). | Alloxan | O-linked N-acetylglucosamine (GlcNAc) transferase | Homo sapiens |
| 2 | In isolated pancreatic islets, alloxan almost completely blocked both glucosamine-induced and STZ-induced protein O-GlcNAcylation, suggesting that alloxan indeed was inhibiting (OGT). | Alloxan | O-linked N-acetylglucosamine (GlcNAc) transferase | Homo sapiens |
| 3 | In order to show definitively that alloxan was inhibiting OGT activity, recombinant OGT was incubated with 0-10 mM alloxan, and OGT activity was measured directly by quantitating UDP-[(3)H]-GlcNAc incorporation into the recombinant protein substrate, nucleoporin p62. | Alloxan | O-linked N-acetylglucosamine (GlcNAc) transferase | Homo sapiens |
| 4 | In order to show definitively that alloxan was inhibiting OGT activity, recombinant OGT was incubated with 0-10 mM alloxan, and OGT activity was measured directly by quantitating UDP-[(3)H]-GlcNAc incorporation into the recombinant protein substrate, nucleoporin p62. | Alloxan | O-linked N-acetylglucosamine (GlcNAc) transferase | Homo sapiens |
| 5 | In order to show definitively that alloxan was inhibiting OGT activity, recombinant OGT was incubated with 0-10 mM alloxan, and OGT activity was measured directly by quantitating UDP-[(3)H]-GlcNAc incorporation into the recombinant protein substrate, nucleoporin p62. | Alloxan | O-linked N-acetylglucosamine (GlcNAc) transferase | Homo sapiens |
| 6 | Under these conditions, OGT activity was completely inhibited by 1 mM alloxan with half-maximal inhibition achieved at a concentration of 0.1 mM alloxan. | Alloxan | O-linked N-acetylglucosamine (GlcNAc) transferase | Homo sapiens |
| 7 | Under these conditions, OGT activity was completely inhibited by 1 mM alloxan with half-maximal inhibition achieved at a concentration of 0.1 mM alloxan. | Alloxan | O-linked N-acetylglucosamine (GlcNAc) transferase | Homo sapiens |
| 8 | Together, these data demonstrate that alloxan is an inhibitor of OGT, and as such, is the first OGT inhibitor described. | Alloxan | O-linked N-acetylglucosamine (GlcNAc) transferase | Homo sapiens |
| 9 | Together, these data demonstrate that alloxan is an inhibitor of OGT, and as such, is the first OGT inhibitor described. | Alloxan | O-linked N-acetylglucosamine (GlcNAc) transferase | Homo sapiens |