Title : Nucleotide binding to human uncoupling protein-2 refolded from bacterial inclusion bodies.

Pub. Date : 2002 Sep 1

PMID : 12030845






1 Functional Relationships(s)
Download
Sentence
Compound Name
Protein Name
Organism
1 These data indicate that: UCP2 (a) is at least partially refolded from sarkosyl-solubilized bacterial inclusion bodies by a two-step treatment with C12E9 detergent and hydroxyapatite; (b) binds purine and pyrimidine nucleoside triphosphates with low micromolar affinity; (c) binds GDP with the same affinity as GDP inhibits superoxide-stimulated uncoupling by kidney mitochondria; and (d) exhibits a different nucleotide preference than kidney mitochondria. Superoxides uncoupling protein 2 Homo sapiens