Title : Activation of cellulose membranes with 1,1'-carbonyldiimidazole or 1-cyano-4-dimethylaminopyridinium tetrafluoroborate as a basis for the development of immunosensors.

Pub. Date : 2002 May 15

PMID : 12009691






3 Functional Relationships(s)
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1 For activation two reagents, 1,1"-carbonyldiimidazole (CDI) and 1-cyano-4-dimethylaminopyridinium tetrafluoroborate (CDAP), were compared with respect to the coupling efficiency for glucose oxidase (GOx) and 1,8-diamino-2,6-dioxaoctane. N,N-carbonyldiimidazole hydroxyacid oxidase 1 Homo sapiens
2 We observed 1.5 microg cm(-2) and 0.4 x 10(-4) U cm(-2) GOx with CDI-activated membranes whereas 1.7 microg cm(-2) and 7.2 x 10(-4) U cm(-2) GOx were observed with CDAP-activated membranes. N,N-carbonyldiimidazole hydroxyacid oxidase 1 Homo sapiens
3 We observed 1.5 microg cm(-2) and 0.4 x 10(-4) U cm(-2) GOx with CDI-activated membranes whereas 1.7 microg cm(-2) and 7.2 x 10(-4) U cm(-2) GOx were observed with CDAP-activated membranes. N,N-carbonyldiimidazole hydroxyacid oxidase 1 Homo sapiens