Pub. Date : 2002 Feb 11
PMID : 11904232
4 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
1 | In addition, prior incubation of human placental microsomes with NAD(+) (cofactor) but not cortisol (substrate) resulted in a concentration-dependent increase (EC(50)=8 microM) in 11 beta-HSD2 activity, indicating that binding of NAD(+) to the microsomal 11 beta-HSD2 facilitated the conversion of cortisol to cortisone. | NAD | hydroxysteroid 11-beta dehydrogenase 2 | Homo sapiens |
2 | In addition, prior incubation of human placental microsomes with NAD(+) (cofactor) but not cortisol (substrate) resulted in a concentration-dependent increase (EC(50)=8 microM) in 11 beta-HSD2 activity, indicating that binding of NAD(+) to the microsomal 11 beta-HSD2 facilitated the conversion of cortisol to cortisone. | NAD | hydroxysteroid 11-beta dehydrogenase 2 | Homo sapiens |
3 | In addition, prior incubation of human placental microsomes with NAD(+) (cofactor) but not cortisol (substrate) resulted in a concentration-dependent increase (EC(50)=8 microM) in 11 beta-HSD2 activity, indicating that binding of NAD(+) to the microsomal 11 beta-HSD2 facilitated the conversion of cortisol to cortisone. | NAD | hydroxysteroid 11-beta dehydrogenase 2 | Homo sapiens |
4 | Thus, this finding substantiates the previously proposed concept that a compulsorily ordered ternary complex mechanism may operate for 11 beta-HSD2, with NAD(+) binding first, followed by a conformational change allowing cortisol binding with high affinity. | NAD | hydroxysteroid 11-beta dehydrogenase 2 | Homo sapiens |