Title : Allosteric regulation provides a molecular mechanism for preferential utilization of the fully assembled dolichol-linked oligosaccharide by the yeast oligosaccharyltransferase.

Pub. Date : 2001 Oct 9

PMID : 11580295






2 Functional Relationships(s)
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1 Allosteric regulation provides a molecular mechanism for preferential utilization of the fully assembled dolichol-linked oligosaccharide by the yeast oligosaccharyltransferase. dolichol-linked oligosaccharide dolichyl-diphosphooligosaccharide--protein glycosyltransferase non-catalytic subunit Homo sapiens
2 Here, the steady-state kinetic properties of the OST were reinvestigated using a proteoliposome assay system consisting of the purified yeast enzyme, near-homogeneous preparations of a dolichol-linked oligosaccharide (Glc(3)Man(9)GlcNAc(2)-PP-Dol or Man(9)GlcNAc(2)-PP-Dol) and an (125)I-labeled tripeptide as the acceptor substrate. dolichol-linked oligosaccharide dolichyl-diphosphooligosaccharide--protein glycosyltransferase non-catalytic subunit Homo sapiens