Title : Affinity purification of Csk protein tyrosine kinase based on its catalytic requirement for divalent metal cations.

Pub. Date : 2001 Feb

PMID : 11162381






3 Functional Relationships(s)
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Protein Name
Organism
1 When bacterial cell lysate containing overexpressed GST-Csk was applied to a column of Zn2+-iminodiacetic acid immobilized to agarose, Csk was specifically retained by the column. Sepharose C-terminal Src kinase Homo sapiens
2 When bacterial cell lysate containing overexpressed GST-Csk was applied to a column of Zn2+-iminodiacetic acid immobilized to agarose, Csk was specifically retained by the column. Sepharose C-terminal Src kinase Homo sapiens
3 The 1 M imidazole-eluted fraction contained pure Csk that had a specific activity similar to the enzyme purified by a glutathione-agarose affinity column. Sepharose C-terminal Src kinase Homo sapiens