Title : Paraffin section storage and immunohistochemistry. Effects of time, temperature, fixation, and retrieval protocol with emphasis on p53 protein and MIB1 antigen.

Pub. Date : 2000 Mar

PMID : 10937051






1 Functional Relationships(s)
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1 Boric acid was used for antigen retrieval on sections stored for 12 weeks at 20 degrees C. For both p53 protein and MIB1 antigen, this resulted in an extent and intensity of immunostaining equal to or higher than (MIB1) that obtained in freshly cut sections, using citrate buffer. Citric Acid MIB E3 ubiquitin protein ligase 1 Homo sapiens