Title : Histidine-607 and histidine-643 provide important interactions for metal support of catalysis in phosphodiesterase-5.

Pub. Date : 2000 Aug 8

PMID : 10924156






3 Functional Relationships(s)
Download
Sentence
Compound Name
Protein Name
Organism
1 In cGMP-binding cGMP-specific PDE (PDE5), we showed previously that point mutation of nine of these profoundly decreases k(cat) when the assay is conducted in the presence of Mg(2+); seven of these are in the prototypical metal-binding motifs A and B (HX(3)HX(n)()E) that we identified earlier. Magnesium phosphodiesterase 5A Homo sapiens
2 In cGMP-binding cGMP-specific PDE (PDE5), we showed previously that point mutation of nine of these profoundly decreases k(cat) when the assay is conducted in the presence of Mg(2+); seven of these are in the prototypical metal-binding motifs A and B (HX(3)HX(n)()E) that we identified earlier. Magnesium phosphodiesterase 5A Homo sapiens
3 This report shows that mutation of either His-607 (A motif) or His-643 (B motif) to alanine profoundly diminishes support of PDE catalysis by Mn(2+) or Mg(2+), but mutation of His-647 in B motif or of Glu in either motif does not. Magnesium phosphodiesterase 5A Homo sapiens