Title : Purification and kinetic analysis of eIF2B from Saccharomyces cerevisiae.

Pub. Date : 2000 Aug 25

PMID : 10852917






1 Functional Relationships(s)
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1 Values for K(m) and V(max) were determined to be 12.2 nm and 250.7 fmol/min, respectively, at 0 degrees C. The calculated turnover number (K(cat)) of 43.2 pmol of GDP released per min/pmol of eIF2B at 30 degrees C is approximately 1 order of magnitude lower than values previously reported for the mammalian factor. Guanosine Diphosphate eukaryotic translation initiation factor 2B subunit delta Homo sapiens