Title : Use of chemical chaperones in the yeast Saccharomyces cerevisiae to enhance heterologous membrane protein expression: high-yield expression and purification of human P-glycoprotein.

Pub. Date : 2000 Apr 1

PMID : 10729188






1 Functional Relationships(s)
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Compound Name
Protein Name
Organism
1 Polyhistidine-tagged P-gp was purified by metal affinity chromatography and reconstituted into proteoliposomes in milligram quantities and its ATPase activity was characterized. Metals ATP binding cassette subfamily B member 1 Homo sapiens