Title : Inhibition of NF-kappaB-DNA binding by mercuric ion: utility of the non-thiol reductant, tris(2-carboxyethyl)phosphine hydrochloride (TCEP), on detection of impaired NF-kappaB-DNA binding by thiol-directed agents.

Pub. Date : 2000 Feb

PMID : 10699356






3 Functional Relationships(s)
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Sentence
Compound Name
Protein Name
Organism
1 Mercuric ion (Hg(2+)), a potent thiol inhibitor, prevents expression of nuclear factor kappaB (NF-kappaB) by mercaptide bond formation with a critical cysteine moiety (cys(62)) on the p50 subunit required for DNA binding. Cysteine nuclear factor kappa B subunit 1 Homo sapiens
2 Mercuric ion (Hg(2+)), a potent thiol inhibitor, prevents expression of nuclear factor kappaB (NF-kappaB) by mercaptide bond formation with a critical cysteine moiety (cys(62)) on the p50 subunit required for DNA binding. Cysteine nuclear factor kappa B subunit 1 Homo sapiens
3 NF-kappaB-DNA binding is typically measured in reaction mixtures in which dithiothreitol (DTT) or other thiol reductants are used to maintain cys(62) in the reduced state. Cysteine nuclear factor kappa B subunit 1 Homo sapiens