Title : A new enzyme electrode for quantification of salicylic acid in a FIA system.

Pub. Date : 1999 Feb

PMID : 10698572






1 Functional Relationships(s)
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1 This work presents an amperometric biosensor incorporated into a flow configuration comprising salicylate hydroxylase that catalyses the irreversible hydroxylation of salicylate to catechol in the presence of NADH and molecular oxygen, and tyrosinase that further oxidises catechol giving o-quinone which is electrochemically reduced at -100 mV vs. Ag/AgCl yielding catechol and entering the catalytic oxidation and electrochemical reduction cycling which results in signal amplification and, consequently, low limits of detection. Oxygen tyrosinase Homo sapiens