Pub. Date : 1999 Jul 9
PMID : 10391884
4 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Thiols mediate superoxide-dependent NADH modification of glyceraldehyde-3-phosphate dehydrogenase. | Sulfhydryl Compounds | glyceraldehyde-3-phosphate dehydrogenase | Homo sapiens |
| 2 | We now demonstrate that in contrast to NO-mediated attachment of NAD, covalent attachment of NADH to GAPDH proceeds in the presence of low molecular weight thiols, independent of NO. | Sulfhydryl Compounds | glyceraldehyde-3-phosphate dehydrogenase | Homo sapiens |
| 3 | Lys-C digestion of GAPDH, followed by peptide isolation by high performance liquid chromatography, matrix-assisted laser desorption ionization time-of-flight analysis, and Edman sequencing, demonstrated that NADH attachment occurred at Cys-149, the active-site thiol. | Sulfhydryl Compounds | glyceraldehyde-3-phosphate dehydrogenase | Homo sapiens |
| 4 | Thus, linkage of GAPDH to NADH, in contrast to NAD, occurs in the presence of thiol, is independent of NO, and is mediated by superoxide. | Sulfhydryl Compounds | glyceraldehyde-3-phosphate dehydrogenase | Homo sapiens |