Title : The size and curvature of anionic covesicle substrate affects the catalytic action of cytosolic phospholipase A2.

Pub. Date : 1999 May 15

PMID : 10328818






1 Functional Relationships(s)
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1 Since DMPM has an intrinsic active site dissociation constant at least 330 times larger than that of PAPC, the optimum conditions for conducting kinetic and mechanistic analyses of cPLA2 with this covesicle substrate is one in which cPLA2 is assayed in the presence of glycerol and with fusion-inducing concentrations of calcium. 1-O-hexadecyl-2-arachidonyl-sn-glycero-3-phosphocholine phospholipase A2 group IVA Homo sapiens