Title : Nucleotide binding to the G12V-mutant of Cdc42 investigated by X-ray diffraction and fluorescence spectroscopy: two different nucleotide states in one crystal.

Pub. Date : 1999 Apr

PMID : 10211824






3 Functional Relationships(s)
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1 The 2.5 A crystal structure of the full length human placental isoform of the Gly12 to Val mutant Cdc42 protein (Cdc42(G12V)) bound to both GDP/Mg2+ and GDPNH2 (guanosine-5"-diphospho-beta-amidate) is reported. magnesium ion cell division cycle 42 Homo sapiens
2 The 2.5 A crystal structure of the full length human placental isoform of the Gly12 to Val mutant Cdc42 protein (Cdc42(G12V)) bound to both GDP/Mg2+ and GDPNH2 (guanosine-5"-diphospho-beta-amidate) is reported. magnesium ion cell division cycle 42 Homo sapiens
3 The amino group of GDPNH2 drastically reduces the affinity to Cdc42 in comparison with that of GDP, causes the loss of the Mg2+ ion, and apparently also increases the conformational flexibility of the protein as seen in the crystal. magnesium ion cell division cycle 42 Homo sapiens