Title : High yield refolding and purification process for recombinant human interleukin-6 expressed in Escherichia coli.

Pub. Date : 1999 Feb 5

PMID : 10099541






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1 The amount of dimeric hIL-6s, thought to be purification artifacts, was decreased by optimizing the salt concentrations of the loading materials in the ion-exchange chromatography, and gradually removing organic solvents from the collected fractions of the preparative reverse-phase HPLC. Salts interleukin 6 Homo sapiens