Pub. Date : 1993 Jan
PMID : 8416996
11 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | We have previously shown that two serine residues present in two conserved regions of the bovine cation-independent mannose 6-phosphate receptor (CI-MPR) cytoplasmic domain are phosphorylated in vivo (residues 2421 and 2492 of the full length bovine CI-MPR precursor). | Serine | insulin like growth factor 2 receptor | Bos taurus |
| 2 | We have previously shown that two serine residues present in two conserved regions of the bovine cation-independent mannose 6-phosphate receptor (CI-MPR) cytoplasmic domain are phosphorylated in vivo (residues 2421 and 2492 of the full length bovine CI-MPR precursor). | Serine | insulin like growth factor 2 receptor | Bos taurus |
| 3 | We have previously shown that two serine residues present in two conserved regions of the bovine cation-independent mannose 6-phosphate receptor (CI-MPR) cytoplasmic domain are phosphorylated in vivo (residues 2421 and 2492 of the full length bovine CI-MPR precursor). | Serine | insulin like growth factor 2 receptor | Bos taurus |
| 4 | In this study, we have used CHO cells to investigate the phosphorylation state of these two serines along the different steps of the CI-MPR exocytic and endocytic recycling pathways. | Serine | insulin like growth factor 2 receptor | Bos taurus |
| 5 | Transport and phosphorylation of the CI-MPR in the biosynthetic pathway were examined using deoxymannojirimycin (dMM), a specific inhibitor of the cis-Golgi processing enzyme alpha-mannosidase I which leads to the accumulation of N-linked high mannose oligosaccharides on glycoproteins. | 1-Deoxynojirimycin | insulin like growth factor 2 receptor | Bos taurus |
| 6 | Transport and phosphorylation of the CI-MPR in the biosynthetic pathway were examined using deoxymannojirimycin (dMM), a specific inhibitor of the cis-Golgi processing enzyme alpha-mannosidase I which leads to the accumulation of N-linked high mannose oligosaccharides on glycoproteins. | dimethylmyleran | insulin like growth factor 2 receptor | Bos taurus |
| 7 | Transport and phosphorylation of the CI-MPR in the biosynthetic pathway were examined using deoxymannojirimycin (dMM), a specific inhibitor of the cis-Golgi processing enzyme alpha-mannosidase I which leads to the accumulation of N-linked high mannose oligosaccharides on glycoproteins. | n-linked high mannose oligosaccharides | insulin like growth factor 2 receptor | Bos taurus |
| 8 | Upon removal of dMM, normal processing to complex-type oligosaccharides (galactosylation and then sialylation) occurs on the newly synthesized glycoproteins, including the CI-MPR which could then be purified and analyzed on lectin affinity columns. | dimethylmyleran | insulin like growth factor 2 receptor | Bos taurus |
| 9 | Upon removal of dMM, normal processing to complex-type oligosaccharides (galactosylation and then sialylation) occurs on the newly synthesized glycoproteins, including the CI-MPR which could then be purified and analyzed on lectin affinity columns. | Oligosaccharides | insulin like growth factor 2 receptor | Bos taurus |
| 10 | Phosphorylation of the newly synthesized CI-MPR was concomitant with the sialylation of its oligosaccharides and appeared as a major albeit transient modification. | Oligosaccharides | insulin like growth factor 2 receptor | Bos taurus |
| 11 | Collectively, these results indicate that phosphorylation of the two serines in the CI-MPR cytoplasmic domain is associated with a single step of transport of its recycling pathways and occurs when this receptor is in the trans-Golgi network and/or has left this compartment via clathrin-coated vesicles. | Serine | insulin like growth factor 2 receptor | Bos taurus |