Title : Interrelations of secondary structure stability and DNA-binding affinity in the bacteriophage SPO1-encoded type II DNA-binding protein TF1.

Pub. Date : 1994 Feb 11

PMID : 8107099






2 Functional Relationships(s)
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Compound Name
Protein Name
Organism
1 TF1, a homodimeric DNA-binding and -bending protein with a preference for hydroxymethyluracil-containing DNA is the Bacillus subtilis-encoded homolog of the bacterial HU proteins and of the E. coli integration host factor. 5-hydroxymethyluracil TF1 Bacillus phage SPO1
2 The double mutant protein TF1-G15I32 binds to a preferred site in hydroxymethyluracil-containing DNA 40 times more tightly, denatures at higher temperature (delta tm = 21 degrees C), and also exchanges subunits much more slowly than does the wild-type protein. 5-hydroxymethyluracil TF1 Bacillus phage SPO1