Pub. Date : 1994 Oct
PMID : 7881184
7 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Using oligosaccharide substrate (Glc1Man9GlcNAc or Man9GlcNAc), the medium of cells transformed with this plasmid showed an increase in alpha-mannosidase activity that was directly related to the increase in cell density, whereas no alpha-mannosidase activity was detected in cells transformed with vector alone. | Oligosaccharides | alpha-mannosidase | Saccharomyces cerevisiae S288C |
| 2 | Using oligosaccharide substrate (Glc1Man9GlcNAc or Man9GlcNAc), the medium of cells transformed with this plasmid showed an increase in alpha-mannosidase activity that was directly related to the increase in cell density, whereas no alpha-mannosidase activity was detected in cells transformed with vector alone. | Oligosaccharides | alpha-mannosidase | Saccharomyces cerevisiae S288C |
| 3 | Using oligosaccharide substrate (Glc1Man9GlcNAc or Man9GlcNAc), the medium of cells transformed with this plasmid showed an increase in alpha-mannosidase activity that was directly related to the increase in cell density, whereas no alpha-mannosidase activity was detected in cells transformed with vector alone. | glc1man9glcnac | alpha-mannosidase | Saccharomyces cerevisiae S288C |
| 4 | Using oligosaccharide substrate (Glc1Man9GlcNAc or Man9GlcNAc), the medium of cells transformed with this plasmid showed an increase in alpha-mannosidase activity that was directly related to the increase in cell density, whereas no alpha-mannosidase activity was detected in cells transformed with vector alone. | mannosyl(9)-N-acetylglucosamine | alpha-mannosidase | Saccharomyces cerevisiae S288C |
| 5 | Using oligosaccharide substrate (Glc1Man9GlcNAc or Man9GlcNAc), the medium of cells transformed with this plasmid showed an increase in alpha-mannosidase activity that was directly related to the increase in cell density, whereas no alpha-mannosidase activity was detected in cells transformed with vector alone. | mannosyl(9)-N-acetylglucosamine | alpha-mannosidase | Saccharomyces cerevisiae S288C |
| 6 | SDS-PAGE of the medium showed the presence of a doublet of 63 and 60 kDa that was revealed by Coomassie Blue staining and by Western blotting with antibodies to the endogenous solubilized alpha-mannosidase. | Sodium Dodecyl Sulfate | alpha-mannosidase | Saccharomyces cerevisiae S288C |
| 7 | The recombinant alpha-mannosidase was present in the medium at a level of approximately 1 mg/l and was purified in a single step by chromatography on S-Sepharose. | Sepharose | alpha-mannosidase | Saccharomyces cerevisiae S288C |