Title : Superiority of ABTS over Trinder reagent as chromogen in highly sensitive peroxidase assays for enzyme linked immunoadsorbent assay.

Pub. Date : 1980 Jun

PMID : 6159451






2 Functional Relationships(s)
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1 In contrast, with suitable temperature and concentrations of H2O2 and ABTS, chromophore production continues in this system for at least 20 h. In an Enzyme Linked Immunoadsorbent Assay for antibodies to myelin basic protein the use of the ABTS/H2O2 substrate system described here gives an assay 14 times more sensitive than the maximum possible with Trinder reagent. 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid myelin basic protein Equus caballus
2 In contrast, with suitable temperature and concentrations of H2O2 and ABTS, chromophore production continues in this system for at least 20 h. In an Enzyme Linked Immunoadsorbent Assay for antibodies to myelin basic protein the use of the ABTS/H2O2 substrate system described here gives an assay 14 times more sensitive than the maximum possible with Trinder reagent. 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid myelin basic protein Equus caballus