Title : Development of a radioimmunoassay for quantitation of calregulin in bovine tissues.

Pub. Date : 1986 Mar 11

PMID : 3964661






3 Functional Relationships(s)
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1 Experimental conditions are described for a convenient and simple one-step method for radioimmunoassay (RIA) of the calcium binding protein calregulin [Waisman, D.M., Salimath, B.P., & Anderson, M.J. (1985) J. Biol. Calcium calreticulin Bos taurus
2 The radioimmunoassay utilizes 125I-labeled calregulin and pansorbin cells (Staphylococcus aureus) coated with rabbit anti-calregulin antibody. Iodine-125 calreticulin Bos taurus
3 Gel permeation chromatography of bovine brain 100 000 g supernatant in the presence or absence of calcium has resolved a single peak of calregulin by RIA. Calcium calreticulin Bos taurus