Title : ATP-Dependent Protease ClpP and Its Subunits ClpA, ClpB, and ClpX Involved in the Field Bismerthiazol Resistance in Xanthomonas oryzae pv. oryzae.

Pub. Date : 2021 Nov

PMID : 33973800






9 Functional Relationships(s)
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1 ATP-dependent protease ClpP and its subunits ClpA, ClpB, and ClpX involved in the field bismerthiazol-resistance in Xanthomonas oryzae pv. bismerthiazol clpP Oryza sativa
2 Here, qPCR was applied to detect the fold expression of ATP-dependent proteases, ClpP and its subunits, which cope with stresses including bactericides in bismerthiazol-resistant strains and their parental susceptible WT strain (ZJ173). bismerthiazol clpP Oryza sativa
3 Results showed that the expression of ClpP and its subunits were all higher in bismerthiazol-resistant strains than that in ZJ173. bismerthiazol clpP Oryza sativa
4 ClpP and its subunits were overexpressed in Xoo in this study, the higher expression of these genes increased sensitivity in vitro and enhanced resistance in vivo to bismerthiazol. bismerthiazol clpP Oryza sativa
5 Inhibition of bismerthiazol to exopolysaccharide production, biofilm, and motility was also reduced in ClpP and its subunit"s overexpression mutants of Xoo. bismerthiazol clpP Oryza sativa
6 The deletion mutants of ClpP and its subunits in ZJ173 abated pathogenicity, biofilm production, swimming ability, exopolysaccharide production, and growth in low-nutrient environments. exopolysaccharide clpP Oryza sativa
7 Moreover, ClpP and its subunits may act downstream of the histidine utilization pathway, which could be inhibited by bismerthiazol in Xoo. Histidine clpP Oryza sativa
8 Moreover, ClpP and its subunits may act downstream of the histidine utilization pathway, which could be inhibited by bismerthiazol in Xoo. bismerthiazol clpP Oryza sativa
9 Taken together, our results indicated that ClpP and its subunits of Xoo influenced the resistance to bismerthiazol. bismerthiazol clpP Oryza sativa