Pub. Date : 1988 Sep
PMID : 3044889
6 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | To evaluate possible mechanisms by which insulin inhibits hepatic apolipoprotein B (apoB) secretion, we incubated primary cultures of rat hepatocytes with sodium orthovanadate, a phosphotyrosine phosphatase inhibitor and insulin-mimetic agent. | Sodium orthovanadate | apolipoprotein B | Rattus norvegicus |
| 2 | Vanadate (10 microM) and insulin (10 nM) inhibited the medium accumulation of apoB (secretion) by 21 and 37%, respectively, without increasing intracellular apoB. | Vanadates | apolipoprotein B | Rattus norvegicus |
| 3 | Unlike insulin, vanadate, at a concentration that inhibited apoB secretion (10 microM), had no effect on intracellular lipogenesis, inhibited the secretion of newly synthesized hepatic proteins, and had a delayed onset and termination of action on inhibition of apoB secretion. | Vanadates | apolipoprotein B | Rattus norvegicus |
| 4 | Unlike insulin, vanadate, at a concentration that inhibited apoB secretion (10 microM), had no effect on intracellular lipogenesis, inhibited the secretion of newly synthesized hepatic proteins, and had a delayed onset and termination of action on inhibition of apoB secretion. | Vanadates | apolipoprotein B | Rattus norvegicus |
| 5 | In conclusion, our data indicate that vanadate mimics insulin action in hepatocytes with regard to the inhibition of medium accumulation of apoB. | Vanadates | apolipoprotein B | Rattus norvegicus |
| 6 | These data are consistent with the hypothesis that inhibition of apoB secretion may be secondary to an increase in phosphotyrosine content at its site of synthesis. | Phosphotyrosine | apolipoprotein B | Rattus norvegicus |