Title : A novel, sensitive assay for O6-methyl- and O6-ethylguanine in DNA, based on repair by the enzyme O6-alkylguanine-DNA-alkyltransferase in competition with an oligonucleotide containing O6-methylguanine.

Pub. Date : 1989 Dec 15

PMID : 2684406






10 Functional Relationships(s)
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1 A novel, sensitive assay for O6-methyl- and O6-ethylguanine in DNA, based on repair by the enzyme O6-alkylguanine-DNA-alkyltransferase in competition with an oligonucleotide containing O6-methylguanine. o6-methyl- and o6-ethylguanine O-6-methylguanine-DNA methyltransferase Rattus norvegicus
2 A novel, sensitive assay for O6-methyl- and O6-ethylguanine in DNA, based on repair by the enzyme O6-alkylguanine-DNA-alkyltransferase in competition with an oligonucleotide containing O6-methylguanine. Oligonucleotides O-6-methylguanine-DNA methyltransferase Rattus norvegicus
3 A novel, sensitive assay for O6-methyl- and O6-ethylguanine in DNA, based on repair by the enzyme O6-alkylguanine-DNA-alkyltransferase in competition with an oligonucleotide containing O6-methylguanine. O-(6)-methylguanine O-6-methylguanine-DNA methyltransferase Rattus norvegicus
4 It is based on the use of the suicide repair enzyme O6-alkylguanine-DNA-alkyltransferase (AGT) to repair such adducts in DNA in competition with an oligonucleotide containing a single residue of O6-methylguanine, end labeled to high specific activity. Oligonucleotides O-6-methylguanine-DNA methyltransferase Rattus norvegicus
5 It is based on the use of the suicide repair enzyme O6-alkylguanine-DNA-alkyltransferase (AGT) to repair such adducts in DNA in competition with an oligonucleotide containing a single residue of O6-methylguanine, end labeled to high specific activity. Oligonucleotides O-6-methylguanine-DNA methyltransferase Rattus norvegicus
6 It is based on the use of the suicide repair enzyme O6-alkylguanine-DNA-alkyltransferase (AGT) to repair such adducts in DNA in competition with an oligonucleotide containing a single residue of O6-methylguanine, end labeled to high specific activity. O-(6)-methylguanine O-6-methylguanine-DNA methyltransferase Rattus norvegicus
7 It is based on the use of the suicide repair enzyme O6-alkylguanine-DNA-alkyltransferase (AGT) to repair such adducts in DNA in competition with an oligonucleotide containing a single residue of O6-methylguanine, end labeled to high specific activity. O-(6)-methylguanine O-6-methylguanine-DNA methyltransferase Rattus norvegicus
8 The stoichiometric mode of action of AGT results in decreased amounts of oligonucleotide being repaired in the presence of increasing levels of adducts in the competing DNA. Oligonucleotides O-6-methylguanine-DNA methyltransferase Rattus norvegicus
9 In view of its relatively wide spectrum of alkyl group specificity, use of AGT from rat liver permits the determination of both O6-methyl- and O6-ethylguanine (detection limits, 0.8 fmol and 3 fmol, respectively). o6-methyl- and o6-ethylguanine O-6-methylguanine-DNA methyltransferase Rattus norvegicus
10 On the other hand, the restricted specificity of Escherichia coli AGT to repair of O6-methylguanine makes the assay based on it specific for this type of lesion (detection limit, 0.5 fmol). O-(6)-methylguanine O-6-methylguanine-DNA methyltransferase Rattus norvegicus