Pub. Date : 1989 Dec 15
PMID : 2684406
10 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | A novel, sensitive assay for O6-methyl- and O6-ethylguanine in DNA, based on repair by the enzyme O6-alkylguanine-DNA-alkyltransferase in competition with an oligonucleotide containing O6-methylguanine. | o6-methyl- and o6-ethylguanine | O-6-methylguanine-DNA methyltransferase | Rattus norvegicus |
| 2 | A novel, sensitive assay for O6-methyl- and O6-ethylguanine in DNA, based on repair by the enzyme O6-alkylguanine-DNA-alkyltransferase in competition with an oligonucleotide containing O6-methylguanine. | Oligonucleotides | O-6-methylguanine-DNA methyltransferase | Rattus norvegicus |
| 3 | A novel, sensitive assay for O6-methyl- and O6-ethylguanine in DNA, based on repair by the enzyme O6-alkylguanine-DNA-alkyltransferase in competition with an oligonucleotide containing O6-methylguanine. | O-(6)-methylguanine | O-6-methylguanine-DNA methyltransferase | Rattus norvegicus |
| 4 | It is based on the use of the suicide repair enzyme O6-alkylguanine-DNA-alkyltransferase (AGT) to repair such adducts in DNA in competition with an oligonucleotide containing a single residue of O6-methylguanine, end labeled to high specific activity. | Oligonucleotides | O-6-methylguanine-DNA methyltransferase | Rattus norvegicus |
| 5 | It is based on the use of the suicide repair enzyme O6-alkylguanine-DNA-alkyltransferase (AGT) to repair such adducts in DNA in competition with an oligonucleotide containing a single residue of O6-methylguanine, end labeled to high specific activity. | Oligonucleotides | O-6-methylguanine-DNA methyltransferase | Rattus norvegicus |
| 6 | It is based on the use of the suicide repair enzyme O6-alkylguanine-DNA-alkyltransferase (AGT) to repair such adducts in DNA in competition with an oligonucleotide containing a single residue of O6-methylguanine, end labeled to high specific activity. | O-(6)-methylguanine | O-6-methylguanine-DNA methyltransferase | Rattus norvegicus |
| 7 | It is based on the use of the suicide repair enzyme O6-alkylguanine-DNA-alkyltransferase (AGT) to repair such adducts in DNA in competition with an oligonucleotide containing a single residue of O6-methylguanine, end labeled to high specific activity. | O-(6)-methylguanine | O-6-methylguanine-DNA methyltransferase | Rattus norvegicus |
| 8 | The stoichiometric mode of action of AGT results in decreased amounts of oligonucleotide being repaired in the presence of increasing levels of adducts in the competing DNA. | Oligonucleotides | O-6-methylguanine-DNA methyltransferase | Rattus norvegicus |
| 9 | In view of its relatively wide spectrum of alkyl group specificity, use of AGT from rat liver permits the determination of both O6-methyl- and O6-ethylguanine (detection limits, 0.8 fmol and 3 fmol, respectively). | o6-methyl- and o6-ethylguanine | O-6-methylguanine-DNA methyltransferase | Rattus norvegicus |
| 10 | On the other hand, the restricted specificity of Escherichia coli AGT to repair of O6-methylguanine makes the assay based on it specific for this type of lesion (detection limit, 0.5 fmol). | O-(6)-methylguanine | O-6-methylguanine-DNA methyltransferase | Rattus norvegicus |