Title : Mutations of Arabidopsis TBL32 and TBL33 Affect Xylan Acetylation and Secondary Wall Deposition.

Pub. Date : 2016

PMID : 26745802






8 Functional Relationships(s)
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Protein Name
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1 Here, we demonstrate the functional roles of TBL32 and TBL33, two ESK1 close homologs, in acetyl substitutions of xylan. Xylans TRICHOME BIREFRINGENCE-LIKE 33 Arabidopsis thaliana
2 Simultaneous mutations of TBL32 and TBL33 resulted in a significant reduction in xylan acetyl content and endoxylanase digestion of the mutant xylan released GlcA-substituted xylooligomers without acetyl groups. Glucuronic Acid TRICHOME BIREFRINGENCE-LIKE 33 Arabidopsis thaliana
3 Structural analysis of xylan revealed that the tbl32 tbl33 mutant had a nearly complete loss of 3-O-acetylated, 2-O-GlcA-substituted xylosyl residues. Xylans TRICHOME BIREFRINGENCE-LIKE 33 Arabidopsis thaliana
4 Structural analysis of xylan revealed that the tbl32 tbl33 mutant had a nearly complete loss of 3-O-acetylated, 2-O-GlcA-substituted xylosyl residues. 3-o TRICHOME BIREFRINGENCE-LIKE 33 Arabidopsis thaliana
5 Structural analysis of xylan revealed that the tbl32 tbl33 mutant had a nearly complete loss of 3-O-acetylated, 2-O-GlcA-substituted xylosyl residues. 2-o-glca TRICHOME BIREFRINGENCE-LIKE 33 Arabidopsis thaliana
6 Simultaneous mutations of TBL32, TBL33 and ESK1 resulted in a severe reduction in xylan acetyl level down to 15% of that of the wild type, and concomitantly, severely collapsed vessels and stunted plant growth. xylan acetyl TRICHOME BIREFRINGENCE-LIKE 33 Arabidopsis thaliana
7 In particular, the S2 layer of secondary walls in xylem vessels of tbl33 esk1 and tbl32 tbl33 esk1 exhibited an altered structure, indicating abnormal assembly of secondary wall polymers. Polymers TRICHOME BIREFRINGENCE-LIKE 33 Arabidopsis thaliana
8 In particular, the S2 layer of secondary walls in xylem vessels of tbl33 esk1 and tbl32 tbl33 esk1 exhibited an altered structure, indicating abnormal assembly of secondary wall polymers. Polymers TRICHOME BIREFRINGENCE-LIKE 33 Arabidopsis thaliana