Title : Upstream activating sequences that are shared by two divergently transcribed operons mediate cAMP-CRP regulation of pilus-adhesin in Escherichia coli.

Pub. Date : 1989 Nov

PMID : 2575704






8 Functional Relationships(s)
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1 Upstream activating sequences that are shared by two divergently transcribed operons mediate cAMP-CRP regulation of pilus-adhesin in Escherichia coli. Cyclic AMP catabolite gene activator protein Escherichia coli
2 Transcription of the genes encoding pilus-adhesin of serotype F13 in digalactoside-binding Escherichia coli required activation by the cAMP-CRP complex. digalactoside catabolite gene activator protein Escherichia coli
3 Transcription of the genes encoding pilus-adhesin of serotype F13 in digalactoside-binding Escherichia coli required activation by the cAMP-CRP complex. Cyclic AMP catabolite gene activator protein Escherichia coli
4 Analysis of protein-DNA interaction in vitro showed that CRP bound in a cAMP-dependent manner to a sequence located 0.2 kb upstream of the point of transcription initiation of the pilus subunit operon. Cyclic AMP catabolite gene activator protein Escherichia coli
5 The cAMP-CRP activation included, in addition to the main pilus operon, the oppositely oriented operon encoding the Papl regulatory protein. Cyclic AMP catabolite gene activator protein Escherichia coli
6 Thus the cAMP-CRP complex and PapB might act in concert and indirectly promote pili synthesis by stimulating expression of the Papl positive regulator. Cyclic AMP catabolite gene activator protein Escherichia coli
7 The results of trans-complementation experiments and analyses using lacZ operon fusion derivatives showed that the cAMP-CRP activation also operated directly in cis on the pilus subunit operon. Cyclic AMP catabolite gene activator protein Escherichia coli
8 The implications for possible mechanisms of transcriptional activation by the cAMP-CRP complex at this novel location between the two oppositely oriented operons are discussed. Cyclic AMP catabolite gene activator protein Escherichia coli