Pub. Date : 2014 Nov
PMID : 25280745
7 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | In this study, we engineered CEN.PK2-1C, a leucine auxotrophic strain having a LEU2 gene mutation, for the production of isobutanol and 3-methyl-1-butanol. | isopentyl alcohol | 3-isopropylmalate dehydrogenase | Saccharomyces cerevisiae S288C |
| 2 | The resulting strain overexpressing LEU2 and LEU4 (D578Y) , a feedback inhibition-insensitive mutant of LEU4, showed a 34-fold increase in 3-methyl-1-butanol synthesis compared with CEN.PK2-1C control strain, producing 765.7 mg/L 3-methyl-1-butanol. | isopentyl alcohol | 3-isopropylmalate dehydrogenase | Saccharomyces cerevisiae S288C |
| 3 | The resulting strain overexpressing LEU2 and LEU4 (D578Y) , a feedback inhibition-insensitive mutant of LEU4, showed a 34-fold increase in 3-methyl-1-butanol synthesis compared with CEN.PK2-1C control strain, producing 765.7 mg/L 3-methyl-1-butanol. | isopentyl alcohol | 2-isopropylmalate synthase LEU4 | Saccharomyces cerevisiae S288C |
| 4 | The resulting strain overexpressing LEU2 and LEU4 (D578Y) , a feedback inhibition-insensitive mutant of LEU4, showed a 34-fold increase in 3-methyl-1-butanol synthesis compared with CEN.PK2-1C control strain, producing 765.7 mg/L 3-methyl-1-butanol. | isopentyl alcohol | 2-isopropylmalate synthase LEU4 | Saccharomyces cerevisiae S288C |
| 5 | The resulting strain overexpressing LEU2 and LEU4 (D578Y) , a feedback inhibition-insensitive mutant of LEU4, showed a 34-fold increase in 3-methyl-1-butanol synthesis compared with CEN.PK2-1C control strain, producing 765.7 mg/L 3-methyl-1-butanol. | isopentyl alcohol | 3-isopropylmalate dehydrogenase | Saccharomyces cerevisiae S288C |
| 6 | The resulting strain overexpressing LEU2 and LEU4 (D578Y) , a feedback inhibition-insensitive mutant of LEU4, showed a 34-fold increase in 3-methyl-1-butanol synthesis compared with CEN.PK2-1C control strain, producing 765.7 mg/L 3-methyl-1-butanol. | isopentyl alcohol | 2-isopropylmalate synthase LEU4 | Saccharomyces cerevisiae S288C |
| 7 | The resulting strain overexpressing LEU2 and LEU4 (D578Y) , a feedback inhibition-insensitive mutant of LEU4, showed a 34-fold increase in 3-methyl-1-butanol synthesis compared with CEN.PK2-1C control strain, producing 765.7 mg/L 3-methyl-1-butanol. | isopentyl alcohol | 2-isopropylmalate synthase LEU4 | Saccharomyces cerevisiae S288C |