Title : [Construction and investigation of a recombinant eukaryotic expression vector for expressing the ORF3 protein of hepatitis E virus in BHK-21 fibroblasts].

Pub. Date : 2014 Jul

PMID : 25203800






1 Functional Relationships(s)
Download
Sentence
Compound Name
Protein Name
Organism
1 The recombinant plasmid was then transfected into BHK-21 cells via the Lipofectamine 2000 reagent; the subsequent ORF3 gene overexpression was confirmed by RT-PCR and the protein expression and location was detected by Western blotting and immunofluorescence assay.Results TThe pDsRed-Monomer-N1-ORF3 recombinant plasmid was successfully constructed. Lipofectamine ankyrin repeat, SAM and basic leucine zipper domain containing 1 Homo sapiens