Title : Insulin modulation of hepatic synthesis and secretion of apolipoprotein B by rat hepatocytes.

Pub. Date : 1990 May 25

PMID : 2187873






4 Functional Relationships(s)
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1 Radioactivity incorporation into apoBH and apoBL, the higher and lower molecular weight forms, was assessed after immunoprecipitation of detergent-solubilized cells and media and separation of the apoB forms using sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Sodium Dodecyl Sulfate apolipoprotein B Rattus norvegicus
2 Radioactivity incorporation into apoBH and apoBL, the higher and lower molecular weight forms, was assessed after immunoprecipitation of detergent-solubilized cells and media and separation of the apoB forms using sodium dodecyl sulfate-polyacrylamide gel electrophoresis. polyacrylamide apolipoprotein B Rattus norvegicus
3 Results demonstrate that 1) insulin inhibits synthesis of apoB from [35S]methionine, 2) insulin stimulates degradation of freshly translated apoB favoring apoBH over apoBL, and 3) an intracellular pool of apoB, primarily apoBL, exists that is largely unaffected by insulin. Sulfur-35 apolipoprotein B Rattus norvegicus
4 Results demonstrate that 1) insulin inhibits synthesis of apoB from [35S]methionine, 2) insulin stimulates degradation of freshly translated apoB favoring apoBH over apoBL, and 3) an intracellular pool of apoB, primarily apoBL, exists that is largely unaffected by insulin. Methionine apolipoprotein B Rattus norvegicus