Title : Monitoring of fatty aldehyde dehydrogenase by formation of pyrenedecanoic acid from pyrenedecanal.

Pub. Date : 2010 Jun

PMID : 19965611






6 Functional Relationships(s)
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1 Monitoring of fatty aldehyde dehydrogenase by formation of pyrenedecanoic acid from pyrenedecanal. pyrenedecanoic acid aldehyde dehydrogenase 3 family member A2 Homo sapiens
2 Monitoring of fatty aldehyde dehydrogenase by formation of pyrenedecanoic acid from pyrenedecanal. pyrenedecanal aldehyde dehydrogenase 3 family member A2 Homo sapiens
3 Fatty aldehyde dehydrogenase (EC 1.2.1.48) converts long-chain fatty aldehydes to the corresponding acids. long-chain fatty aldehydes aldehyde dehydrogenase 3 family member A2 Homo sapiens
4 Using a fluorescent aldehyde, pyrenedecanal, and HPLC with fluorescence detection, we developed a novel method to monitor fatty aldehyde dehydrogenase activity by quantification of the product pyrenedecanoic acid together with the substrate pyrenedecanal and possible side products, such as aldehyde adducts. Aldehydes aldehyde dehydrogenase 3 family member A2 Homo sapiens
5 Using a fluorescent aldehyde, pyrenedecanal, and HPLC with fluorescence detection, we developed a novel method to monitor fatty aldehyde dehydrogenase activity by quantification of the product pyrenedecanoic acid together with the substrate pyrenedecanal and possible side products, such as aldehyde adducts. pyrenedecanoic acid aldehyde dehydrogenase 3 family member A2 Homo sapiens
6 Thus, monitoring of pyrenedecanoic acid formation from pyrenedecanal by HPLC with fluorescence detection provides a robust and sensitive method for determination of fatty aldehyde dehydrogenase activity. pyrenedecanoic acid aldehyde dehydrogenase 3 family member A2 Homo sapiens