Title : Renaturation of recombinant Ro 60 autoantigen by calcium ions on PVDF membrane.

Pub. Date : 2009

PMID : 19378069






13 Functional Relationships(s)
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Compound Name
Protein Name
Organism
1 Renaturation of recombinant Ro 60 autoantigen by calcium ions on PVDF membrane. Calcium Ro60, Y RNA binding protein Homo sapiens
2 Renaturation of recombinant Ro 60 autoantigen by calcium ions on PVDF membrane. polyvinylidene fluoride Ro60, Y RNA binding protein Homo sapiens
3 In our earlier studies we found that calcium was involved in the protein-protein interaction observed between Ro 60 multiple antigenic peptides (MAPs) and Ro 60 autoantigen. Calcium Ro60, Y RNA binding protein Homo sapiens
4 In our earlier studies we found that calcium was involved in the protein-protein interaction observed between Ro 60 multiple antigenic peptides (MAPs) and Ro 60 autoantigen. Calcium Ro60, Y RNA binding protein Homo sapiens
5 Since calcium was found to bind Ro 60 MAPs we hypothesized that it would renature human recombinant Ro 60 on a protein blot. Calcium Ro60, Y RNA binding protein Homo sapiens
6 Since calcium was found to bind Ro 60 MAPs we hypothesized that it would renature human recombinant Ro 60 on a protein blot. Calcium Ro60, Y RNA binding protein Homo sapiens
7 As hypothesized antibodies to Ro 60 bound significantly higher to the recombinant Ro antigen that was incubated with calcium compared with that incubated without calcium on a polyvinyline fluoride (PVDF) blot. Calcium Ro60, Y RNA binding protein Homo sapiens
8 As hypothesized antibodies to Ro 60 bound significantly higher to the recombinant Ro antigen that was incubated with calcium compared with that incubated without calcium on a polyvinyline fluoride (PVDF) blot. polyvinyline fluoride Ro60, Y RNA binding protein Homo sapiens
9 As hypothesized antibodies to Ro 60 bound significantly higher to the recombinant Ro antigen that was incubated with calcium compared with that incubated without calcium on a polyvinyline fluoride (PVDF) blot. polyvinylidene fluoride Ro60, Y RNA binding protein Homo sapiens
10 Since the immmunological epitopes of Ro 60 are mainly conformational, we believe that calcium induced a more native tertiary structure in recombinant Ro 60 autoantigen -following blotting to a PVDF membrane. Calcium Ro60, Y RNA binding protein Homo sapiens
11 Since the immmunological epitopes of Ro 60 are mainly conformational, we believe that calcium induced a more native tertiary structure in recombinant Ro 60 autoantigen -following blotting to a PVDF membrane. Calcium Ro60, Y RNA binding protein Homo sapiens
12 Since the immmunological epitopes of Ro 60 are mainly conformational, we believe that calcium induced a more native tertiary structure in recombinant Ro 60 autoantigen -following blotting to a PVDF membrane. polyvinylidene fluoride Ro60, Y RNA binding protein Homo sapiens
13 Since the immmunological epitopes of Ro 60 are mainly conformational, we believe that calcium induced a more native tertiary structure in recombinant Ro 60 autoantigen -following blotting to a PVDF membrane. polyvinylidene fluoride Ro60, Y RNA binding protein Homo sapiens