Pub. Date : 2008 Mar 15
PMID : 18241852
15 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | CYP26A1 knockout embryonic stem cells exhibit reduced differentiation and growth arrest in response to retinoic acid. | Tretinoin | cytochrome P450 family 26 subfamily A member 1 | Homo sapiens |
| 2 | CYP26A1, a cytochrome P450 enzyme, metabolizes all-trans-retinoic acid (RA) into polar metabolites, e.g. 4-oxo-RA and 4-OH-RA. | Tretinoin | cytochrome P450 family 26 subfamily A member 1 | Homo sapiens |
| 3 | CYP26A1, a cytochrome P450 enzyme, metabolizes all-trans-retinoic acid (RA) into polar metabolites, e.g. 4-oxo-RA and 4-OH-RA. | Tretinoin | cytochrome P450 family 26 subfamily A member 1 | Homo sapiens |
| 4 | CYP26A1, a cytochrome P450 enzyme, metabolizes all-trans-retinoic acid (RA) into polar metabolites, e.g. 4-oxo-RA and 4-OH-RA. | 4-oxoretinoic acid | cytochrome P450 family 26 subfamily A member 1 | Homo sapiens |
| 5 | CYP26A1, a cytochrome P450 enzyme, metabolizes all-trans-retinoic acid (RA) into polar metabolites, e.g. 4-oxo-RA and 4-OH-RA. | 4-oh-ra | cytochrome P450 family 26 subfamily A member 1 | Homo sapiens |
| 6 | CYP26a1(-/-) ES cells had a 11.0+/-3.2-fold higher intracellular RA concentration than Wt ES cells after RA treatment for 48 h. RA-treated CYP26A1(-/-) ES cells exhibited 2-3 fold higher mRNA levels of Hoxa1, a primary RA target gene, than Wt ES cells. | Tretinoin | cytochrome P450 family 26 subfamily A member 1 | Homo sapiens |
| 7 | CYP26a1(-/-) ES cells had a 11.0+/-3.2-fold higher intracellular RA concentration than Wt ES cells after RA treatment for 48 h. RA-treated CYP26A1(-/-) ES cells exhibited 2-3 fold higher mRNA levels of Hoxa1, a primary RA target gene, than Wt ES cells. | Tretinoin | cytochrome P450 family 26 subfamily A member 1 | Homo sapiens |
| 8 | CYP26a1(-/-) ES cells had a 11.0+/-3.2-fold higher intracellular RA concentration than Wt ES cells after RA treatment for 48 h. RA-treated CYP26A1(-/-) ES cells exhibited 2-3 fold higher mRNA levels of Hoxa1, a primary RA target gene, than Wt ES cells. | Tretinoin | cytochrome P450 family 26 subfamily A member 1 | Homo sapiens |
| 9 | CYP26a1(-/-) ES cells had a 11.0+/-3.2-fold higher intracellular RA concentration than Wt ES cells after RA treatment for 48 h. RA-treated CYP26A1(-/-) ES cells exhibited 2-3 fold higher mRNA levels of Hoxa1, a primary RA target gene, than Wt ES cells. | Tretinoin | cytochrome P450 family 26 subfamily A member 1 | Homo sapiens |
| 10 | CYP26a1(-/-) ES cells had a 11.0+/-3.2-fold higher intracellular RA concentration than Wt ES cells after RA treatment for 48 h. RA-treated CYP26A1(-/-) ES cells exhibited 2-3 fold higher mRNA levels of Hoxa1, a primary RA target gene, than Wt ES cells. | Tretinoin | cytochrome P450 family 26 subfamily A member 1 | Homo sapiens |
| 11 | CYP26a1(-/-) ES cells had a 11.0+/-3.2-fold higher intracellular RA concentration than Wt ES cells after RA treatment for 48 h. RA-treated CYP26A1(-/-) ES cells exhibited 2-3 fold higher mRNA levels of Hoxa1, a primary RA target gene, than Wt ES cells. | Tretinoin | cytochrome P450 family 26 subfamily A member 1 | Homo sapiens |
| 12 | Despite increased intracellular RA levels, CYP26a1(-/-) ES cells were more resistant than Wt ES cells to RA-induced proliferation arrest. | Tretinoin | cytochrome P450 family 26 subfamily A member 1 | Homo sapiens |
| 13 | Microarray analyses revealed that RA-treated CYP26A1(-/-) ES cells exhibited lower mRNA levels than Wt ES cells for genes involved in differentiation, particularly in neural (Epha4, Pmp22, Nrp1, Gap43, Ndn) and smooth muscle differentiation (Madh3, Nrp1, Tagln Calponin, Caldesmon1). | Tretinoin | cytochrome P450 family 26 subfamily A member 1 | Homo sapiens |
| 14 | In contrast, genes involved in the stress response (e.g. Tlr2, Stk2, Fcgr2b, Bnip3, Pdk1) were expressed at higher levels in CYP26A1(-/-) than in Wt ES cells without RA. | Tretinoin | cytochrome P450 family 26 subfamily A member 1 | Homo sapiens |
| 15 | Collectively, our results show that CYP26A1 activity regulates intracellular RA levels, cell proliferation, transcriptional regulation of primary RA target genes, and ES cell differentiation to parietal endoderm. | Tretinoin | cytochrome P450 family 26 subfamily A member 1 | Homo sapiens |