Pub. Date : 2008 Mar 4
PMID : 18232643
6 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Five generations of directed evolution resulted in yeast pyruvate decarboxylase 1 (Pdc1) variants with improved activity for 1 mM pyruvate at pH 7.5 in the presence of phosphate. | Pyruvic Acid | indolepyruvate decarboxylase 1 | Saccharomyces cerevisiae S288C |
| 2 | Five generations of directed evolution resulted in yeast pyruvate decarboxylase 1 (Pdc1) variants with improved activity for 1 mM pyruvate at pH 7.5 in the presence of phosphate. | Phosphates | indolepyruvate decarboxylase 1 | Saccharomyces cerevisiae S288C |
| 3 | Five generations of directed evolution resulted in yeast pyruvate decarboxylase 1 (Pdc1) variants with improved activity for 1 mM pyruvate at pH 7.5 in the presence of phosphate. | Phosphates | indolepyruvate decarboxylase 1 | Saccharomyces cerevisiae S288C |
| 4 | In comparison with native Pdc1, 5LS30 had the substrate concentration required for half-saturation reduced by almost 3-fold at pH 7.5 and the phosphate inhibition reduced by 4-fold at pH 6.0. | Phosphates | indolepyruvate decarboxylase 1 | Saccharomyces cerevisiae S288C |
| 5 | Curiously, the optimal temperature for Pdc1 activity was found to be dependent upon pyruvate concentration. | Pyruvic Acid | indolepyruvate decarboxylase 1 | Saccharomyces cerevisiae S288C |
| 6 | In 1 mM pyruvate, native Pdc1 performed optimally at 30 degrees C and 5LS30 at 40 degrees C, whereas in 25 mM pyruvate native activity peaked at 45 degrees C and 5LS30 at 55 degrees C. Two screening processes were developed for directed evolution of Pdc1 expressed in Escherichia coli: colony screening and culture screening. | Pyruvic Acid | indolepyruvate decarboxylase 1 | Saccharomyces cerevisiae S288C |