Title : Engineering of alpha1-antitrypsin variants selective for subtilisin-like proprotein convertases PACE4 and PC6: importance of the P2' residue in stable complex formation of the serpin with proprotein convertase.

Pub. Date : 2007 Apr

PMID : 17351018






3 Functional Relationships(s)
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Compound Name
Protein Name
Organism
1 Substituting Arg-Arg-Arg-Arg for Ala-Val-Pro-Met(352) at P4-P1 and Ala for Leu(354) at P2" created a potent PACE4- and PC6-specific inhibitor. arg-arg-arg-arg proprotein convertase subtilisin/kexin type 6 Homo sapiens
2 Substituting Arg-Arg-Arg-Arg for Ala-Val-Pro-Met(352) at P4-P1 and Ala for Leu(354) at P2" created a potent PACE4- and PC6-specific inhibitor. ala-val-pro-met proprotein convertase subtilisin/kexin type 6 Homo sapiens
3 This variant (RRRRSA) formed an SDS- and heat-stable serpin/proteinase complex with PACE4 or PC6 and inhibited both enzyme activities. Sodium Dodecyl Sulfate proprotein convertase subtilisin/kexin type 6 Homo sapiens