Title : Application of silver staining to the rapid typing of the polymorphism of HLA-DQ alleles by enzymatic amplification and allele-specific restriction fragment length polymorphism.

Pub. Date : 1991 Apr

PMID : 1712722






3 Functional Relationships(s)
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1 Application of silver staining to the rapid typing of the polymorphism of HLA-DQ alleles by enzymatic amplification and allele-specific restriction fragment length polymorphism. Silver major histocompatibility complex, class II, DQ alpha 1 Homo sapiens
2 Digestion of the PCR products by endonucleases, followed by their size-separation on polyacrylamide gels and visualization by silver staining, allowed us to define the HLA-DQ alleles of the genomic DNA. Silver major histocompatibility complex, class II, DQ alpha 1 Homo sapiens
3 Thus, silver staining in combination with PCR and allele-specific restriction fragment length polymorphism provides a simple, safe, and rapid method for accurate definition of HLA-DQ alleles at the nucleotide level in the clinical typing laboratory. Silver major histocompatibility complex, class II, DQ alpha 1 Homo sapiens