Pub. Date : 2006 May
PMID : 16929849
8 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | [Purification of cadmium ion binding metallothionein-3 by proteinase digestion on affinity chromatographic column]. | Cadmium | metallothionein 3 | Homo sapiens |
| 2 | Fusion protein GST-Cd2+-hMT-3 was expressed after isopropyl-beta-D-thiogalactopyranoside (IPTG) induction and addition of 0.1 mmol/L CdSO4 into the culture medium, and mainly existed in cellular soluble fraction as revealed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis. | Isopropyl Thiogalactoside | metallothionein 3 | Homo sapiens |
| 3 | Fusion protein GST-Cd2+-hMT-3 was expressed after isopropyl-beta-D-thiogalactopyranoside (IPTG) induction and addition of 0.1 mmol/L CdSO4 into the culture medium, and mainly existed in cellular soluble fraction as revealed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis. | Isopropyl Thiogalactoside | metallothionein 3 | Homo sapiens |
| 4 | Fusion protein GST-Cd2+-hMT-3 was expressed after isopropyl-beta-D-thiogalactopyranoside (IPTG) induction and addition of 0.1 mmol/L CdSO4 into the culture medium, and mainly existed in cellular soluble fraction as revealed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis. | cadmium sulfate | metallothionein 3 | Homo sapiens |
| 5 | Fusion protein GST-Cd2+-hMT-3 was expressed after isopropyl-beta-D-thiogalactopyranoside (IPTG) induction and addition of 0.1 mmol/L CdSO4 into the culture medium, and mainly existed in cellular soluble fraction as revealed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis. | Sodium Dodecyl Sulfate | metallothionein 3 | Homo sapiens |
| 6 | Fusion protein GST-Cd2+-hMT-3 was expressed after isopropyl-beta-D-thiogalactopyranoside (IPTG) induction and addition of 0.1 mmol/L CdSO4 into the culture medium, and mainly existed in cellular soluble fraction as revealed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis. | polyacrylamide | metallothionein 3 | Homo sapiens |
| 7 | Fusion protein GST-Cd2+-hMT-3 was expressed after isopropyl-beta-D-thiogalactopyranoside (IPTG) induction and addition of 0.1 mmol/L CdSO4 into the culture medium, and mainly existed in cellular soluble fraction as revealed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis. | Sodium Dodecyl Sulfate | metallothionein 3 | Homo sapiens |
| 8 | SDS-PAGE, amino acid composition and inductively coupled plasma atomic emission spectrometer (ICP-AES) analysis showed that the relative molecular mass of Cd2+-hMT-3 is about 7 000, with a purity above 90%. | Sodium Dodecyl Sulfate | metallothionein 3 | Homo sapiens |