Title : Single amino acid substitutions in the cAMP receptor protein specifically abolish regulation by the CytR repressor in Escherichia coli.

Pub. Date : 1991 Jun 1

PMID : 1647022






7 Functional Relationships(s)
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1 Promoters in Escherichia coli that are negatively regulated by the CytR repressor are also activated by the cAMP receptor protein (CRP) complexed to cAMP; as a characteristic, these promoters encode tandem binding sites for cAMP-CRP. Cyclic AMP catabolite gene activator protein Escherichia coli
2 Promoters in Escherichia coli that are negatively regulated by the CytR repressor are also activated by the cAMP receptor protein (CRP) complexed to cAMP; as a characteristic, these promoters encode tandem binding sites for cAMP-CRP. Cyclic AMP catabolite gene activator protein Escherichia coli
3 Promoters in Escherichia coli that are negatively regulated by the CytR repressor are also activated by the cAMP receptor protein (CRP) complexed to cAMP; as a characteristic, these promoters encode tandem binding sites for cAMP-CRP. Cyclic AMP catabolite gene activator protein Escherichia coli
4 Promoters in Escherichia coli that are negatively regulated by the CytR repressor are also activated by the cAMP receptor protein (CRP) complexed to cAMP; as a characteristic, these promoters encode tandem binding sites for cAMP-CRP. Cyclic AMP catabolite gene activator protein Escherichia coli
5 Promoters in Escherichia coli that are negatively regulated by the CytR repressor are also activated by the cAMP receptor protein (CRP) complexed to cAMP; as a characteristic, these promoters encode tandem binding sites for cAMP-CRP. Cyclic AMP catabolite gene activator protein Escherichia coli
6 In one such promoter, deoP2, CytR binds to the region between the tandem CRP binding sites with a relatively low affinity; in the presence of cAMP-CRP, however, the repressor and activator bind cooperatively to the DNA. Cyclic AMP catabolite gene activator protein Escherichia coli
7 These results strongly indicate that cooperative DNA binding of CytR and cAMP-CRP is achieved through protein-protein interactions. Cyclic AMP catabolite gene activator protein Escherichia coli