Pub. Date : 2005 May 15
PMID : 15899835
18 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | The human multidrug resistance protein MRP5 transports folates and can mediate cellular resistance against antifolates. | Folic Acid | ATP binding cassette subfamily C member 5 | Homo sapiens |
| 2 | Here we show that MRP5/ABCC5 is also an antifolate and folate exporter based on the following evidence: (a) Using membrane vesicles from HEK293 cells, we show that MRP5 transports both MTX (KM = 1.3 mmol/L and VMAX = 780 pmol per mg protein per minute) and folic acid (KM = 1.0 mmol/L and VMAX = 875 pmol per mg protein per minute). | Methotrexate | ATP binding cassette subfamily C member 5 | Homo sapiens |
| 3 | Here we show that MRP5/ABCC5 is also an antifolate and folate exporter based on the following evidence: (a) Using membrane vesicles from HEK293 cells, we show that MRP5 transports both MTX (KM = 1.3 mmol/L and VMAX = 780 pmol per mg protein per minute) and folic acid (KM = 1.0 mmol/L and VMAX = 875 pmol per mg protein per minute). | Methotrexate | ATP binding cassette subfamily C member 5 | Homo sapiens |
| 4 | Here we show that MRP5/ABCC5 is also an antifolate and folate exporter based on the following evidence: (a) Using membrane vesicles from HEK293 cells, we show that MRP5 transports both MTX (KM = 1.3 mmol/L and VMAX = 780 pmol per mg protein per minute) and folic acid (KM = 1.0 mmol/L and VMAX = 875 pmol per mg protein per minute). | Methotrexate | ATP binding cassette subfamily C member 5 | Homo sapiens |
| 5 | Here we show that MRP5/ABCC5 is also an antifolate and folate exporter based on the following evidence: (a) Using membrane vesicles from HEK293 cells, we show that MRP5 transports both MTX (KM = 1.3 mmol/L and VMAX = 780 pmol per mg protein per minute) and folic acid (KM = 1.0 mmol/L and VMAX = 875 pmol per mg protein per minute). | Folic Acid | ATP binding cassette subfamily C member 5 | Homo sapiens |
| 6 | Here we show that MRP5/ABCC5 is also an antifolate and folate exporter based on the following evidence: (a) Using membrane vesicles from HEK293 cells, we show that MRP5 transports both MTX (KM = 1.3 mmol/L and VMAX = 780 pmol per mg protein per minute) and folic acid (KM = 1.0 mmol/L and VMAX = 875 pmol per mg protein per minute). | Folic Acid | ATP binding cassette subfamily C member 5 | Homo sapiens |
| 7 | Here we show that MRP5/ABCC5 is also an antifolate and folate exporter based on the following evidence: (a) Using membrane vesicles from HEK293 cells, we show that MRP5 transports both MTX (KM = 1.3 mmol/L and VMAX = 780 pmol per mg protein per minute) and folic acid (KM = 1.0 mmol/L and VMAX = 875 pmol per mg protein per minute). | Folic Acid | ATP binding cassette subfamily C member 5 | Homo sapiens |
| 8 | MRP5 also transports MTX-glu2 (KM = 0.7 mmol/L and VMAX = 450 pmol per mg protein per minute) but not MTX-glu3. | mtx-glu2 | ATP binding cassette subfamily C member 5 | Homo sapiens |
| 9 | (b) Both accumulation of total [3H]MTX and of MTX polyglutamates were significantly reduced in MRP5 overexpressing cells. | Tritium | ATP binding cassette subfamily C member 5 | Homo sapiens |
| 10 | (b) Both accumulation of total [3H]MTX and of MTX polyglutamates were significantly reduced in MRP5 overexpressing cells. | Methotrexate | ATP binding cassette subfamily C member 5 | Homo sapiens |
| 11 | (b) Both accumulation of total [3H]MTX and of MTX polyglutamates were significantly reduced in MRP5 overexpressing cells. | methotrexate polyglutamate | ATP binding cassette subfamily C member 5 | Homo sapiens |
| 12 | (c) Cell growth inhibition studies with MRP5 transfected HEK293 cells showed that MRP5 conferred high-level resistance (>160-fold) against the antifolates MTX, GW1843, and ZD1694 (raltitrexed) in short-term (4 hours) incubations with high drug concentrations; this resistance was proportional to the MRP5 level. | Methotrexate | ATP binding cassette subfamily C member 5 | Homo sapiens |
| 13 | (c) Cell growth inhibition studies with MRP5 transfected HEK293 cells showed that MRP5 conferred high-level resistance (>160-fold) against the antifolates MTX, GW1843, and ZD1694 (raltitrexed) in short-term (4 hours) incubations with high drug concentrations; this resistance was proportional to the MRP5 level. | Methotrexate | ATP binding cassette subfamily C member 5 | Homo sapiens |
| 14 | (c) Cell growth inhibition studies with MRP5 transfected HEK293 cells showed that MRP5 conferred high-level resistance (>160-fold) against the antifolates MTX, GW1843, and ZD1694 (raltitrexed) in short-term (4 hours) incubations with high drug concentrations; this resistance was proportional to the MRP5 level. | Methotrexate | ATP binding cassette subfamily C member 5 | Homo sapiens |
| 15 | (c) Cell growth inhibition studies with MRP5 transfected HEK293 cells showed that MRP5 conferred high-level resistance (>160-fold) against the antifolates MTX, GW1843, and ZD1694 (raltitrexed) in short-term (4 hours) incubations with high drug concentrations; this resistance was proportional to the MRP5 level. | 1843U89 | ATP binding cassette subfamily C member 5 | Homo sapiens |
| 16 | (c) Cell growth inhibition studies with MRP5 transfected HEK293 cells showed that MRP5 conferred high-level resistance (>160-fold) against the antifolates MTX, GW1843, and ZD1694 (raltitrexed) in short-term (4 hours) incubations with high drug concentrations; this resistance was proportional to the MRP5 level. | 1843U89 | ATP binding cassette subfamily C member 5 | Homo sapiens |
| 17 | (d) MRP5-mediated resistance (8.5- and 2.1-fold) was also found in standard long-term incubations (72 hours) at low concentrations of ZD1694 and GW1843. | raltitrexed | ATP binding cassette subfamily C member 5 | Homo sapiens |
| 18 | (d) MRP5-mediated resistance (8.5- and 2.1-fold) was also found in standard long-term incubations (72 hours) at low concentrations of ZD1694 and GW1843. | 1843U89 | ATP binding cassette subfamily C member 5 | Homo sapiens |