Pub. Date : 2004 Apr
PMID : 15089093
25 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Equine catechol estrogen 4-hydroxyequilenin is a more potent inhibitor of the variant form of catechol-O-methyltransferase. | catechol | catechol O-methyltransferase | Equus caballus |
| 2 | Equine catechol estrogen 4-hydroxyequilenin is a more potent inhibitor of the variant form of catechol-O-methyltransferase. | 4-hydroxy-equilenin | catechol O-methyltransferase | Equus caballus |
| 3 | Catechol-O-methyltransferase (COMT) plays an important role in the inactivation of biologically active and toxic catechols. | Catechols | catechol O-methyltransferase | Equus caballus |
| 4 | Catechol-O-methyltransferase (COMT) plays an important role in the inactivation of biologically active and toxic catechols. | Catechols | catechol O-methyltransferase | Equus caballus |
| 5 | It has been shown that COMT is genetically polymorphic with a wild-type and variant form where a valine has been substituted with a methionine. | Valine | catechol O-methyltransferase | Equus caballus |
| 6 | It has been shown that COMT is genetically polymorphic with a wild-type and variant form where a valine has been substituted with a methionine. | Methionine | catechol O-methyltransferase | Equus caballus |
| 7 | Previously, we showed that 4-hydroxyequilenin (4-OHEN), a cytotoxic/genotoxic equine catechol estrogen metabolite, is both a substrate of COMT and an irreversible inhibitor of the methylation activity of COMT in vitro. | 4-hydroxy-equilenin | catechol O-methyltransferase | Equus caballus |
| 8 | Previously, we showed that 4-hydroxyequilenin (4-OHEN), a cytotoxic/genotoxic equine catechol estrogen metabolite, is both a substrate of COMT and an irreversible inhibitor of the methylation activity of COMT in vitro. | 4-hydroxy-equilenin | catechol O-methyltransferase | Equus caballus |
| 9 | Previously, we showed that 4-hydroxyequilenin (4-OHEN), a cytotoxic/genotoxic equine catechol estrogen metabolite, is both a substrate of COMT and an irreversible inhibitor of the methylation activity of COMT in vitro. | 4-ohen | catechol O-methyltransferase | Equus caballus |
| 10 | Previously, we showed that 4-hydroxyequilenin (4-OHEN), a cytotoxic/genotoxic equine catechol estrogen metabolite, is both a substrate of COMT and an irreversible inhibitor of the methylation activity of COMT in vitro. | 4-ohen | catechol O-methyltransferase | Equus caballus |
| 11 | To further understand the mechanism(s) of the association between the breast cancer risk and the COMT polymorphism, it was of interest to study the effect of the Val/Met polymorphism on COMT-catalyzed catechol estrogen methylation and 4-OHEN-mediated inhibition. | Valine | catechol O-methyltransferase | Equus caballus |
| 12 | To further understand the mechanism(s) of the association between the breast cancer risk and the COMT polymorphism, it was of interest to study the effect of the Val/Met polymorphism on COMT-catalyzed catechol estrogen methylation and 4-OHEN-mediated inhibition. | Methionine | catechol O-methyltransferase | Equus caballus |
| 13 | To further understand the mechanism(s) of the association between the breast cancer risk and the COMT polymorphism, it was of interest to study the effect of the Val/Met polymorphism on COMT-catalyzed catechol estrogen methylation and 4-OHEN-mediated inhibition. | catechol | catechol O-methyltransferase | Equus caballus |
| 14 | To further understand the mechanism(s) of the association between the breast cancer risk and the COMT polymorphism, it was of interest to study the effect of the Val/Met polymorphism on COMT-catalyzed catechol estrogen methylation and 4-OHEN-mediated inhibition. | catechol | catechol O-methyltransferase | Equus caballus |
| 15 | To further understand the mechanism(s) of the association between the breast cancer risk and the COMT polymorphism, it was of interest to study the effect of the Val/Met polymorphism on COMT-catalyzed catechol estrogen methylation and 4-OHEN-mediated inhibition. | 4-ohen | catechol O-methyltransferase | Equus caballus |
| 16 | However, we found that the COMT variant form was more susceptible to 4-OHEN-mediated irreversible inactivation. | 4-ohen | catechol O-methyltransferase | Equus caballus |
| 17 | Electrospray ionization mass spectrometry and SDS-gel analysis of COMT modified by 4-OHEN revealed that inhibition mechanisms include alkylation and/or oxidation of certain amino acids. | Sodium Dodecyl Sulfate | catechol O-methyltransferase | Equus caballus |
| 18 | Electrospray ionization mass spectrometry and SDS-gel analysis of COMT modified by 4-OHEN revealed that inhibition mechanisms include alkylation and/or oxidation of certain amino acids. | 4-ohen | catechol O-methyltransferase | Equus caballus |
| 19 | Furthermore, thermotropic studies indicated that the variant form was more thermolabile, which suggested that the valine to methionine substitution may have changed the secondary/tertiary structure of the variant form of COMT, making it more susceptible to 4-OHEN and heat inactivation. | Valine | catechol O-methyltransferase | Equus caballus |
| 20 | Furthermore, thermotropic studies indicated that the variant form was more thermolabile, which suggested that the valine to methionine substitution may have changed the secondary/tertiary structure of the variant form of COMT, making it more susceptible to 4-OHEN and heat inactivation. | Methionine | catechol O-methyltransferase | Equus caballus |
| 21 | Furthermore, thermotropic studies indicated that the variant form was more thermolabile, which suggested that the valine to methionine substitution may have changed the secondary/tertiary structure of the variant form of COMT, making it more susceptible to 4-OHEN and heat inactivation. | 4-ohen | catechol O-methyltransferase | Equus caballus |
| 22 | These data suggest that 4-OHEN-mediated inhibition of the variant form of COMT in vivo might affect the detoxification efficiency of endogenous and/or exogenous catechol estrogens and play a role in the association between breast cancer risk and COMT polymorphism. | 4-ohen | catechol O-methyltransferase | Equus caballus |
| 23 | These data suggest that 4-OHEN-mediated inhibition of the variant form of COMT in vivo might affect the detoxification efficiency of endogenous and/or exogenous catechol estrogens and play a role in the association between breast cancer risk and COMT polymorphism. | 4-ohen | catechol O-methyltransferase | Equus caballus |
| 24 | These data suggest that 4-OHEN-mediated inhibition of the variant form of COMT in vivo might affect the detoxification efficiency of endogenous and/or exogenous catechol estrogens and play a role in the association between breast cancer risk and COMT polymorphism. | catechol | catechol O-methyltransferase | Equus caballus |
| 25 | These data suggest that 4-OHEN-mediated inhibition of the variant form of COMT in vivo might affect the detoxification efficiency of endogenous and/or exogenous catechol estrogens and play a role in the association between breast cancer risk and COMT polymorphism. | catechol | catechol O-methyltransferase | Equus caballus |