Title : Immunolocalization, quantitation and cellular heterogeneity of apolipoprotein B in rat hepatocytes.

Pub. Date : 1992 Jun

PMID : 1375579






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1 To demonstrate the utility of the technique for quantitative studies, static cell cytofluorometry of brefeldin A-treated cells was performed, demonstrating increases in specific immunofluorescence of apolipoprotein B corresponding closely to results estimated by monoclonal antibody radioimmunoassays of cellular homogenates. Brefeldin A apolipoprotein B Rattus norvegicus
2 Results for brefeldin A-treated cells revealed even clearer delineation of heterogeneity as indicated by frank bimodality of the populations, along with not only higher mean apolipoprotein B levels but also a significantly higher proportion of apolipoprotein B-positive cells than in the control. Brefeldin A apolipoprotein B Rattus norvegicus
3 Results for brefeldin A-treated cells revealed even clearer delineation of heterogeneity as indicated by frank bimodality of the populations, along with not only higher mean apolipoprotein B levels but also a significantly higher proportion of apolipoprotein B-positive cells than in the control. Brefeldin A apolipoprotein B Rattus norvegicus