Title : Biosynthesis and metabolism of dipeptidylpeptidase IV in primary cultured rat hepatocytes and Morris hepatoma 7777 cells.

Pub. Date : 1992 Nov 15

PMID : 1359965






11 Functional Relationships(s)
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1 N-Glycosylation, biosynthesis and degradation of dipeptidylpeptidase IV (EC 3.4.14.5) (DPP IV) were comparatively studied in primary cultured rat hepatocytes and Morris hepatoma 7777 cells (MH 7777 cells). Nitrogen dipeptidylpeptidase 4 Rattus norvegicus
2 DPP IV had a molecular mass of 105 kDa in rat hepatocytes and of 103 kDa in MH 7777 cells as assessed by SDS/PAGE under reducing conditions. Sodium Dodecyl Sulfate dipeptidylpeptidase 4 Rattus norvegicus
3 The precursor was processed to the mature forms of DPP IV, glycosylated with N-glycans mainly of the complex type with a half-life of 20-25 min. n-glycans dipeptidylpeptidase 4 Rattus norvegicus
4 DPP IV molecules were very slowly degraded in hepatocytes as well as in hepatoma cells with half-lives of approximately 45 h. Inhibition of oligosaccharide processing with 1-deoxymannojirimycin led to the formation of DPP IV molecules containing N-glycans of the oligomannosidic type. Oligosaccharides dipeptidylpeptidase 4 Rattus norvegicus
5 DPP IV molecules were very slowly degraded in hepatocytes as well as in hepatoma cells with half-lives of approximately 45 h. Inhibition of oligosaccharide processing with 1-deoxymannojirimycin led to the formation of DPP IV molecules containing N-glycans of the oligomannosidic type. Oligosaccharides dipeptidylpeptidase 4 Rattus norvegicus
6 DPP IV molecules were very slowly degraded in hepatocytes as well as in hepatoma cells with half-lives of approximately 45 h. Inhibition of oligosaccharide processing with 1-deoxymannojirimycin led to the formation of DPP IV molecules containing N-glycans of the oligomannosidic type. 1-Deoxynojirimycin dipeptidylpeptidase 4 Rattus norvegicus
7 DPP IV molecules were very slowly degraded in hepatocytes as well as in hepatoma cells with half-lives of approximately 45 h. Inhibition of oligosaccharide processing with 1-deoxymannojirimycin led to the formation of DPP IV molecules containing N-glycans of the oligomannosidic type. 1-Deoxynojirimycin dipeptidylpeptidase 4 Rattus norvegicus
8 By contrast, inhibition of N-glycosylation with tunicamycin resulted into rapid degradation of non-N-glycosylated DPP IV molecules in both cell types. Nitrogen dipeptidylpeptidase 4 Rattus norvegicus
9 By contrast, inhibition of N-glycosylation with tunicamycin resulted into rapid degradation of non-N-glycosylated DPP IV molecules in both cell types. Tunicamycin dipeptidylpeptidase 4 Rattus norvegicus
10 By contrast, inhibition of N-glycosylation with tunicamycin resulted into rapid degradation of non-N-glycosylated DPP IV molecules in both cell types. Nitrogen dipeptidylpeptidase 4 Rattus norvegicus
11 Non-N-glycosylated DPP IV could not be detected at the cell surface indicating an intracellular proteolytic process soon after biosynthesis. Nitrogen dipeptidylpeptidase 4 Rattus norvegicus