Title : PMA-activated neutrophils decrease pulmonary endothelial ectoenzyme activities in perfused rabbit lungs.

Pub. Date : 1992 Dec

PMID : 1335699






3 Functional Relationships(s)
Download
Sentence
Compound Name
Protein Name
Organism
1 Enzyme activities were estimated from the hydrolysis of the substrates [3H]benzoyl-Phe-Ala-Pro ([3H]BPAP) by ACE and 14C-labeled AMP by NCT during a single transpulmonary passage, using indicator-dilution techniques. [3h]benzoyl-phe-ala-pro angiotensin-converting enzyme Oryctolagus cuniculus
2 Enzyme activities were estimated from the hydrolysis of the substrates [3H]benzoyl-Phe-Ala-Pro ([3H]BPAP) by ACE and 14C-labeled AMP by NCT during a single transpulmonary passage, using indicator-dilution techniques. [3h]bpap angiotensin-converting enzyme Oryctolagus cuniculus
3 At the same time, Km values of BPAP for ACE and AMP for NCT were elevated from 9.2 +/- 2.2 to 19.3 +/- 3 microM and 6.7 +/- 1.2 to 15.1 +/- 3.6 microM, respectively, whereas Amax (product of enzyme mass and rate of product formation, thus an index of perfused microvascular surface area) did not change. bpap angiotensin-converting enzyme Oryctolagus cuniculus