Title : Positive and negative control of ompB transcription in Escherichia coli by cyclic AMP and the cyclic AMP receptor protein.

Pub. Date : 1992 Feb

PMID : 1310090






4 Functional Relationships(s)
Download
Sentence
Compound Name
Protein Name
Organism
1 In vivo primer extension analysis indicated that this control is complex and involves both positive and negative effects by cyclic AMP-CRP on multiple ompB promoters. Cyclic AMP catabolite gene activator protein Escherichia coli
2 In vitro footprinting showed that cyclic AMP-CRP binds to a 34-bp site centered at -53 and at -75 in relation to the start sites of the major transcripts that are inhibited and activated, respectively, by this complex. Cyclic AMP catabolite gene activator protein Escherichia coli
3 Site-directed mutagenesis of the crp binding site provided evidence that this site is necessary for the in vivo regulation of ompB expression by cyclic AMP. Cyclic AMP catabolite gene activator protein Escherichia coli
4 Control of the ompB operon by cyclic AMP-CRP may account for the observed regulation of the formation of OmpF and OmpC by this complex (N. W. Scott and C. R. Harwood, FEMS Microbiol. Cyclic AMP catabolite gene activator protein Escherichia coli