Title : Monoclonal antibodies to Escherichia coli-expressed P46 and P65 membranous proteins for specific immunodetection of Mycoplasma hyopneumoniae in lungs of infected pigs.

Pub. Date : 2003 May

PMID : 12738649






3 Functional Relationships(s)
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Protein Name
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1 Following induction by IPTG (isopropyl-beta-D-thiogalactopyranoside), both GST-P46 and GST-P65(c) recombinant fusion proteins were recovered by disrupting transformed cells by sonication, purified by affinity chromatography, and then cut with thrombin to release the P46 and P65(c) moieties. Isopropyl Thiogalactoside v-rel reticuloendotheliosis viral oncogene homolog A (avian) Mus musculus
2 Following induction by IPTG (isopropyl-beta-D-thiogalactopyranoside), both GST-P46 and GST-P65(c) recombinant fusion proteins were recovered by disrupting transformed cells by sonication, purified by affinity chromatography, and then cut with thrombin to release the P46 and P65(c) moieties. Isopropyl Thiogalactoside v-rel reticuloendotheliosis viral oncogene homolog A (avian) Mus musculus
3 Both anti-P46 and anti-P65(c) MAbs permitted effective detection by indirect immunofluorescence and indirect immunoperoxidase assay of M. hyopneumoniae in, respectively, frozen and formalin-fixed, paraffin-embedded lung sections from pigs that were killed after the 6- to 7-week observation period. Formaldehyde v-rel reticuloendotheliosis viral oncogene homolog A (avian) Mus musculus