Pub. Date : 2002 Jul 16
PMID : 12102636
18 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | The rat organic anion transporter, rOAT3, mediates the transport of organic anions such as p-aminohippurate (PAH) and estrone sulfate as well as the basic compound, cimetidine. | p-Aminohippuric Acid | solute carrier family 22 member 8 | Rattus norvegicus |
| 2 | The rat organic anion transporter, rOAT3, mediates the transport of organic anions such as p-aminohippurate (PAH) and estrone sulfate as well as the basic compound, cimetidine. | p-Aminohippuric Acid | solute carrier family 22 member 8 | Rattus norvegicus |
| 3 | The rat organic anion transporter, rOAT3, mediates the transport of organic anions such as p-aminohippurate (PAH) and estrone sulfate as well as the basic compound, cimetidine. | estrone sulfate | solute carrier family 22 member 8 | Rattus norvegicus |
| 4 | The rat organic anion transporter, rOAT3, mediates the transport of organic anions such as p-aminohippurate (PAH) and estrone sulfate as well as the basic compound, cimetidine. | Cimetidine | solute carrier family 22 member 8 | Rattus norvegicus |
| 5 | In the present study, we examined the role of conserved transmembrane aromatic amino acid residues of rOAT3 in substrate recognition and transport. | Amino Acids, Aromatic | solute carrier family 22 member 8 | Rattus norvegicus |
| 6 | Alanine scanning followed by amino acid replacements was used to construct mutants of rOAT3. | Alanine | solute carrier family 22 member 8 | Rattus norvegicus |
| 7 | We observed that four mutants in transmembrane domain 7 (TMD 7), W334A, F335A, Y341A, and Y342Q, and one mutant in transmembrane domain 8 (TMD 8), F362S, exhibited a less than 2-fold enhanced uptake of PAH and cimetidine in comparison to wild-type rOAT3, which exhibited a 16-fold enhanced uptake of PAH and an 8-fold enhanced uptake of cimetidine. | p-Aminohippuric Acid | solute carrier family 22 member 8 | Rattus norvegicus |
| 8 | We observed that four mutants in transmembrane domain 7 (TMD 7), W334A, F335A, Y341A, and Y342Q, and one mutant in transmembrane domain 8 (TMD 8), F362S, exhibited a less than 2-fold enhanced uptake of PAH and cimetidine in comparison to wild-type rOAT3, which exhibited a 16-fold enhanced uptake of PAH and an 8-fold enhanced uptake of cimetidine. | Cimetidine | solute carrier family 22 member 8 | Rattus norvegicus |
| 9 | We observed that four mutants in transmembrane domain 7 (TMD 7), W334A, F335A, Y341A, and Y342Q, and one mutant in transmembrane domain 8 (TMD 8), F362S, exhibited a less than 2-fold enhanced uptake of PAH and cimetidine in comparison to wild-type rOAT3, which exhibited a 16-fold enhanced uptake of PAH and an 8-fold enhanced uptake of cimetidine. | p-Aminohippuric Acid | solute carrier family 22 member 8 | Rattus norvegicus |
| 10 | We observed that four mutants in transmembrane domain 7 (TMD 7), W334A, F335A, Y341A, and Y342Q, and one mutant in transmembrane domain 8 (TMD 8), F362S, exhibited a less than 2-fold enhanced uptake of PAH and cimetidine in comparison to wild-type rOAT3, which exhibited a 16-fold enhanced uptake of PAH and an 8-fold enhanced uptake of cimetidine. | Cimetidine | solute carrier family 22 member 8 | Rattus norvegicus |
| 11 | We observed that the residues contribute to PAH and cimetidine transport in different ways: the -OH group of Y342, the indole ring of W334, and the aromatic rings of F335, Y341, and F362 are important for PAH and cimetidine transport by rOAT3. | p-Aminohippuric Acid | solute carrier family 22 member 8 | Rattus norvegicus |
| 12 | We observed that the residues contribute to PAH and cimetidine transport in different ways: the -OH group of Y342, the indole ring of W334, and the aromatic rings of F335, Y341, and F362 are important for PAH and cimetidine transport by rOAT3. | Cimetidine | solute carrier family 22 member 8 | Rattus norvegicus |
| 13 | We observed that the residues contribute to PAH and cimetidine transport in different ways: the -OH group of Y342, the indole ring of W334, and the aromatic rings of F335, Y341, and F362 are important for PAH and cimetidine transport by rOAT3. | indole | solute carrier family 22 member 8 | Rattus norvegicus |
| 14 | We observed that the residues contribute to PAH and cimetidine transport in different ways: the -OH group of Y342, the indole ring of W334, and the aromatic rings of F335, Y341, and F362 are important for PAH and cimetidine transport by rOAT3. | p-Aminohippuric Acid | solute carrier family 22 member 8 | Rattus norvegicus |
| 15 | We observed that the residues contribute to PAH and cimetidine transport in different ways: the -OH group of Y342, the indole ring of W334, and the aromatic rings of F335, Y341, and F362 are important for PAH and cimetidine transport by rOAT3. | Cimetidine | solute carrier family 22 member 8 | Rattus norvegicus |
| 16 | These data suggest that there is an aromatic pocket composed mainly of residues in TMD 7 in the translocation pathway of rOAT3, which is important for the transport of PAH and cimetidine. | p-Aminohippuric Acid | solute carrier family 22 member 8 | Rattus norvegicus |
| 17 | These data suggest that there is an aromatic pocket composed mainly of residues in TMD 7 in the translocation pathway of rOAT3, which is important for the transport of PAH and cimetidine. | Cimetidine | solute carrier family 22 member 8 | Rattus norvegicus |
| 18 | Aromatic residues in this pocket may interact directly with substrates of rOAT3 through hydrogen bonds and pi-pi interactions. | Hydrogen | solute carrier family 22 member 8 | Rattus norvegicus |