Pub. Date : 2001 Nov
PMID : 11745748
8 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Thiolation of polycarbophil enhances its inhibition of intestinal brush border membrane bound aminopeptidase N. | calcium polycarbophil | alanyl aminopeptidase, membrane | Sus scrofa |
| 2 | Aminopeptidase N activity was assayed spectrophotometrically using L-leucine p-nitroanilide (leu-pNA) as a synthetic substrate and against the model peptide drug leu-enkp, by high-performance liquid chromatography (HPLC). | H-LEU-PNA | alanyl aminopeptidase, membrane | Sus scrofa |
| 3 | Aminopeptidase N activity was assayed spectrophotometrically using L-leucine p-nitroanilide (leu-pNA) as a synthetic substrate and against the model peptide drug leu-enkp, by high-performance liquid chromatography (HPLC). | L-Leucine-p-nitroanilide | alanyl aminopeptidase, membrane | Sus scrofa |
| 4 | Free cysteine at 6.3 and 63 microM (pH 6) significantly (p < 0.05) inhibited aminopeptidase N activity, and PCP-Cys (0.25% w/v, pH 6) had a significantly (p < 0.05) greater inhibitory effect than PCP on the aminopeptidase N activity towards both substrates. | Cysteine | alanyl aminopeptidase, membrane | Sus scrofa |
| 5 | Free cysteine at 6.3 and 63 microM (pH 6) significantly (p < 0.05) inhibited aminopeptidase N activity, and PCP-Cys (0.25% w/v, pH 6) had a significantly (p < 0.05) greater inhibitory effect than PCP on the aminopeptidase N activity towards both substrates. | pcp-cys | alanyl aminopeptidase, membrane | Sus scrofa |
| 6 | PCP-Cys completely protected leu-enkp against aminopeptidase N activity over a 2-h incubation period, whereas 83 +/- 4 and 60 +/- 7% remained stable in the presence of PCP and buffer only, respectively. | pcp-cys | alanyl aminopeptidase, membrane | Sus scrofa |
| 7 | PCP-Cys completely protected leu-enkp against aminopeptidase N activity over a 2-h incubation period, whereas 83 +/- 4 and 60 +/- 7% remained stable in the presence of PCP and buffer only, respectively. | pcp | alanyl aminopeptidase, membrane | Sus scrofa |
| 8 | Thiolation of PCP increased the stability of leu-enkp against the enzymatic degradation by aminopeptidase N and the intact intestinal mucosa, identifying a promising new excipient for peroral delivery of peptides. | pcp | alanyl aminopeptidase, membrane | Sus scrofa |