Pub. Date : 1998
PMID : 9711577
3 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Human Pgp from the vinblastine-resistant cell line, KB-V1, can be purified by sequential conventional chromatography on DEAE-sepharose CL-6B resin followed by a wheat germ agglutinin column. | Vinblastine | phosphoglycolate phosphatase | Homo sapiens |
| 2 | When this method was used for purification of Pgp from MDR1-transfected NIH 3T3 transfectants (N3-V2400, grown in the presence of 2.4 micrograms/ml vinblastine), the protein was eluted with 0.1 M NaCl from the DEAE-Sepharose CL-6B column as usual. | Vinblastine | phosphoglycolate phosphatase | Homo sapiens |
| 3 | This is further supported by the qualitative demonstration of ATP-dependent vinblastine transport in proteoliposomes reconstituted with pure Pgp (see Fig. | Vinblastine | phosphoglycolate phosphatase | Homo sapiens |